Human galectin-9 ELISA kit (Gal-9) (ab273161)
Key features and details
- Sensitivity: 9 pg/ml
- Range: 15.6 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human galectin-9 ELISA kit (Gal-9)
See all galectin 9/Gal-9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Various 18 = 3.2% Inter-assay Sample n Mean SD CV% 1 345.43pg/ml 15.45 4.5% 2 189.62pg/ml 25.4 13.4% 3 461.78pg/ml 11.51 2.5% 4 249.55pg/ml 13.28 5.3% 5 589.98pg/ml 8.15 1.4% 6 296.4pg/ml 21.38 7.2% -
Sample type
Cell culture supernatant, Serum, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9 pg/ml -
Range
15.6 pg/ml - 500 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Galectin-9 ELISA kit (Gal-9) (ab273161) is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of Galectin-9 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human Galectin-9.
A capture antibody highly specific for Galectin-9 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Galectin-9 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-Galectin-9 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Galectin-9 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Galectin-9 in any sample tested.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody Gal-9 1 x 400µl Gal-9 Coated Microwell strips 1 unit Gal-9 Standard 2 vials HRP Diluent 1 x 23ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 25ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml -
Research areas
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Alternative names
- 36 kDa beta-galactoside-binding lectin
- Ecalectin
- Gal-9
see all -
Database links
- Entrez Gene: 3965 Human
- Omim: 601879 Human
- SwissProt: O00182 Human
- Unigene: 81337 Human
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Human Galectin-9 ELISA kit (Gal-9) standard curve.