Anti-PKC zeta (phospho T560) antibody (ab59412)
Key features and details
- Rabbit polyclonal to PKC zeta (phospho T560)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-PKC zeta (phospho T560) antibody
See all PKC zeta primary antibodies -
Description
Rabbit polyclonal to PKC zeta (phospho T560) -
Host species
Rabbit -
Specificity
Detects endogenous levels of PKC? only when phosphorylated at threonine 560. -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human PKC zeta aa 526-575.
Database link: Q05513 -
Positive control
- WB: Extracts from COS7 cells treated with PMA (125ng/ml, 30mins). IHC-P: Human brain gliomas, human brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins) with phosphopeptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
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Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution
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Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
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ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody, ab59412.