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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC

Anti-PKC zeta (phospho T560) antibody (ab59412)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-PKC zeta (phospho T560) antibody (ab59412)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PKC zeta (phospho T560)
  • Suitable for: WB, ELISA, IHC-P
  • Reacts with: Human, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-PKC zeta (phospho T560) antibody
    See all PKC zeta primary antibodies
  • Description

    Rabbit polyclonal to PKC zeta (phospho T560)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of PKC? only when phosphorylated at threonine 560.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human PKC zeta aa 526-575.
    Database link: Q05513

  • Positive control

    • WB: Extracts from COS7 cells treated with PMA (125ng/ml, 30mins). IHC-P: Human brain gliomas, human brain tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC

Images

  • Western blot - Anti-PKC zeta (phospho T560) antibody (ab59412)
    Western blot - Anti-PKC zeta (phospho T560) antibody (ab59412)
    All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated
    with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from COS7 cells treated
    with PMA (125ng/ml, 30mins) with phosphopeptide

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)

    Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)
    Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
  • ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)
    ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)

    ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.

  • ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)
    ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)

    Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody, ab59412.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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