Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1901(2)] to PKC alpha (phospho S657) - BSA and Azide free
  • Suitable for: Dot blot, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free
    See all PKC alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR1901(2)] to PKC alpha (phospho S657) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects PKC alpha phosphorylated at serine 657.

  • Tested applications

    Suitable for: Dot blot, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250251 is the carrier-free version of ab180848. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250251 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution

    Lane 1 : Untreated C6 (rat glioma) whole cell lysate
    Lane 2 : C6 (rat glioma) treated with 80nM Phorbol-12-myristate-13-acetate, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab180848, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Dot Blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)

    This data was developed using ab180848, the same antibody clone in a different buffer formulation.

    Dot blot analysis of PKC alpha (pS657) phospho peptide (Lane 1) and PKC alpha non-phospho peptide (Lane 2) using ab180848 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution. Blocking and Diluting buffer and concentration: 5% NFDM /TBST. Exposure time: 10 seconds.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    This data was developed using ab180848, the same antibody clone in a different buffer formulation.ab180848 staining PKC alpha (phospho S657) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution

    Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) treatrd with 200ng/ml phorbol-12,13-dibutyrate for 45 minutes, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab180848, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Lysate from Jurkat cells treated with phorbol-12,13-dibutyrate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 77 kDa



    This data was developed using ab180848, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    This data was developed using ab180848, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling PKC alpha (phospho S657) with ab180848. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    This data was developed using ab180848, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human stomach tissue labeling PKC alpha (phospho S657) with ab180848. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)
    Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - BSA and Azide free (ab250251)

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