Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 03, 2021

Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)
Suitable for: WB, IHC-P, Dot blot
Reacts with: Human

Product name

Anti-PKC zeta (phospho T560) antibody [EP2037AY]
See all PKC zeta primary antibodies
Description

Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)
Host species

Rabbit
Specificity

ab62372 detects PKC zeta phosphorylated at Threonine 560.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PKC zeta aa 550-650 (phospho T560). The exact sequence is proprietary.
Positive control
- WB: HeLa cell lysates. IHC-P: Human brain tissue and human breast ductal carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2037AY
Isotype

IgG
Research areas

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

Lane 1 : HeLa cell lysate - treated with Calyculin A
Lane 2 : HeLa cell lysate - treated with Calyculin A and lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

Lane 1 : Hela Cell Lysate (untreated with Calyculin A)
Lane 2 : Hela Cell Lysate (Treated with Calyculin A)

Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

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