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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Oncoproteins/suppressors Tumor suppressors

Anti-Beclin 1 antibody (ab223348)

Anti-Beclin 1 antibody (ab223348)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Beclin 1
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Beclin 1 antibody
    See all Beclin 1 primary antibodies
  • Description

    Rabbit polyclonal to Beclin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Beclin 1 (internal sequence).
    Database link: Q14457

  • Positive control

    • WB: Rat liver lysate; HeLa cell lysate. ICC/IF: SK-N-BE cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab223348)
    Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab223348)

    4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223348 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223348 (D) Merge.

  • Western blot - Anti-Beclin 1 antibody (ab223348)
    Western blot - Anti-Beclin 1 antibody (ab223348)
    Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Predicted band size: 52 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 12 minutes at RT.

  • Western blot - Anti-Beclin 1 antibody (ab223348)
    Western blot - Anti-Beclin 1 antibody (ab223348)
    Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + Rat liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Predicted band size: 52 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 12 minutes at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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