Anti-Caspase-9 antibody [EPR18868] (ab184786)
![Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-251487-anti-caspase-9-antibody-epr18868-western-blot.jpg "Anti-Caspase-9 antibody [EPR18868] (ab184786)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18868] to Caspase-9
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Caspase-9 antibody [EPR18868]
See all Caspase-9 primary antibodies -
Description
Rabbit monoclonal [EPR18868] to Caspase-9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain and heart lysates. IP: NIH/3T3 and C6 treated with 1µM staurosporine for 4h whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18868 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 0.5 hour
Lane 3 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour
Lane 4 : C6 whole cell lysate treated with 1 µm staurosporine for 4 hours
Lane 5 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour, then treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The p39 and p37 subunits were inhibited when the caspase activity was blocked by the caspase inhibitor, Z-VAD-FMK.
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![Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-251486-anti-caspase-9-antibody-epr18868-western-blot.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-251485-anti-caspase-9-antibody-epr18868-western-blot.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18868] (ab184786)All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-251484-anti-caspase-9-antibody-epr18868-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)Caspase 9 was immunoprecipitated from 1mg of NIH/3T3 whole cell lysate (Mouse embyro fibroblast cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h,10ug (Input).
Lane 2: ab184786 IP in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could not be observed even with 3 minutes exposure time.
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![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-251483-anti-caspase-9-antibody-epr18868-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18868] (ab184786)Caspase 9 was immunoprecipitated from 1mg of C6 whole cell lysate (Rat glial tumor cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate treated with 1μM staurosporine for 4h, 10ug (Input).
Lane 2: ab184786 IP in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could be observed with longer exposure time.
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![Anti-Caspase-9 antibody [EPR18868] (ab184786)](https://www.abcam.com/ps/products/184/ab184786/Images/ab184786-6-benefits-of-recombinant-antibodies.png)
Anti-Caspase-9 antibody [EPR18868] (ab184786)
