Anti-Caspase-9 (phospho T125) antibody (ab192815)
Key features and details
- Rabbit polyclonal to Caspase-9 (phospho T125)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-9 (phospho T125) antibody
See all Caspase-9 primary antibodies -
Description
Rabbit polyclonal to Caspase-9 (phospho T125) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Caspase-9 (phospho T125) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R).
Database link: P55211 -
Positive control
- HeLa whole cell lysate (ab150035) and Human lung carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-9 (phospho T125) antibody (ab192815)All lanes : Anti-CDC25 (phospho S916) antibody (ab192816) at 1/500 dilution
Lane 1 : HeLa cell lysate with antigen-specific peptide
Lane 2 : HeLa cell lysate
Predicted band size: 46 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho T125) antibody (ab192815)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Caspase-9 (phospho T125) using ab192815 at a 1/50 dilution, the right panel was treated with blocking peptide.
