Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16790] to Caspase-2L - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free
    See all Caspase-2L primary antibodies

  • Description

    Rabbit monoclonal [EPR16790] to Caspase-2L - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250106 is the carrier-free version of ab179519. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250106 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Immunocytochemistry - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)

    This data was developed using ab179519, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Caspase-2L with ab179519 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab179519 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    All lanes : Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates treated with 1uM staurosporine for 4 hours
    Lane 2 : untreated Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    This data was developed using ab179519, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed MW: 48, 30, 14, 12 kDa.

  • Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    This data was developed using ab179519, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed MW: 48, 30, 12 kDa.

  • Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Western blot - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/10000 dilution + C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    This data was developed using ab179519, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed MW: 48, 12 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    This data was developed using ab179519, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the germ cells of the Human testis was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    This data was developed using ab179519, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the tumor cells of Human lung cancer was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Flow Cytometry - Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    This data was developed using ab179519, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab179519 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)
    Anti-Caspase-2L antibody [EPR16790] - BSA and Azide free (ab250106)

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