Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-5 antibody [EP876Y] (ab40887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP876Y] to Caspase-5
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-5 antibody [EP876Y]
    See all Caspase-5 primary antibodies

  • Description

    Rabbit monoclonal [EP876Y] to Caspase-5

  • Host species

    Rabbit

  • Specificity

    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Caspase-5 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human breast carcinoma. WB: HT-1080 and HeLa cell lysates. IP: HeLa cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP876Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/1000 dilution + HT-1080 (Human fibrosarcoma epithelial cell) prepared in 1%SDS Hot lysis methodwhole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] (ab40887)

    Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (ab40887)

    Purified ab40887 at 1/50 dilution (2µg) immunoprecipitating Caspase-5 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat shock (42? 1h) then recovery for 6h whole cell lysate 10µg
    Lane 2 (+): ab40887 + HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40887 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 47 kDa

  • Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    The cell lysates were prepared in 1%SDS Hot lysis method.

  • Anti-Caspase-5 antibody [EP876Y] (ab40887)
    Anti-Caspase-5 antibody [EP876Y] (ab40887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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