Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells
Overview
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Product name
Free Fatty Acid Uptake Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Adherent cells -
Assay type
Cell-based (quantitative) -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate. This fatty acid uptake assay kit can be performed on any fluorescence microplate reader with a bottom-read mode at Ex/Em = 485/515 nm or FITC channel. The assay can be performed in 96-well or 384-well microtiter plates in a simple mix-and-read procedure, and easily adapted for high throughput screening applications.
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Notes
Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabetes and hepatic steatosis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 10ml DMSO 1 x 100µl TF2-C12 Fatty Acid 1 vial -
Research areas
Images
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Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.
Cells were plated at 50,000 cells/100μL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 ºC, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 μL of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a plate reader using bottom read mode. A – fibroblasts (Control); B – fibroblasts (Insulin); C – adipocytes (Control); D– adipocytes (Insulin).
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Excitation and Emission Spectra of Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)