Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
![Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.com/ps/products/2/ab2739/Images/ab2739-279434-anti-o-linked-n-acetylglucosamine-antibody-rl2-immunofluorescence.jpg "Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)")
Key features and details
- Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-O-Linked N-Acetylglucosamine antibody [RL2]
See all O-Linked N-Acetylglucosamine primary antibodies -
Description
Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex
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Positive control
- ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
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General notes
This antibody clone [RL2] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
RL2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.com/ps/products/2/ab2739/Images/ab2739-232645-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml
Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutesJurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.com/ps/products/2/ab2739/Images/ab2739-232732-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) This image is courtesy of an anonymous AbreviewAll lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution
Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated horse anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.com/ps/products/2/ab2739/Images/ab2739-6300-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).
