Nitric Oxide Assay Kit (Colorimetric) (ab65328)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 nmol/well
Overview
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Product name
Nitric Oxide Assay Kit (Colorimetric)
See all Nitric oxide kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Sensitivity
1 nmol/well -
Range
1 nmol/well - 10 nmol/well -
Assay time
1h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
In the nitric oxide assay protocol:
- the first step converts nitrate to nitrite by using nitrate reductase
- the second step uses Griess Reagent to convert nitrite to a deep purple azo compound.
The amount of the azochromophore accurately reflects nitric oxide amount in samples.Nitric oxide assay protocol summary:
- add samples and standards to wells
- add nitrate reductase and enzyme cofactor and incubate for 1 hr at room temp to convert nitrate to nitrite
- add enhancer and incubate for 10 min at room temp
- add Griess Reagent R1 and Griess Reagent R2
- analyze with microplate reader -
Notes
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327).
For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044.
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 2 x 96 tests Assay Buffer WM 1 x 30ml Enhancer (Lyophilized) Purple 1 vial Enzyme Cofactor (Lyophilized) Blue 1 vial Griess Reagent R1 NM 1 x 10ml Griess Reagent R2 Amber NM 1 x 10ml Microtiter plate 2 units Nitrate Reductase (Lyophilized) Green 1 vial Nitrate Standard (Lyophilized) Yellow 1 vial Nitrite Standard (Lyophilized) Orange 1 vial Plate Cover 2 units -
Research areas
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Relevance
Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.
Images
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Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328) Nam, Eunryel et al., Scientific reports vol. 8,1 7312., Fig 4 , doi:10.1038/s41598-018-25786-xBlood NOx concentration measured with ab65328. Blood NOx concentration after application of the NE with NO. The level of blood NOx concentration increased 3-fold after the application of the NE with NO compared with control group.
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Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)Nitrite and nitrate measured in cell lysates showing quantity (micromol) per 106 cells
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Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)Nitrite and nitrate measured in biologicals showing concentration (micromolar)
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Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies - Nitric Oxide Colorimetric Assay Kit (ab65328)Standard curve (Colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
