Cell Invasion Assay Kit (Collagen I), 24-well, 8 µm (ab235887)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Invasion Assay Kit (Collagen I), 24-well, 8 µm
See all Cell Invasion kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Product overview
This kit provides enough materials for 12 assays, i.e. to run 12 wells of assay, although it comes in a 24 well plate format.
Cell Invasion Assay (Collagen I), 24-well, 8 µm (ab235887) utilizes a Boyden chamber coated with collagen I, where the cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
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Notes
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical & mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 12 tests Cell Dissociation Solution 1 x 6ml Cell Dye 1 vial Cell Invasion Chamber 1 unit Collagen I 1 x 1.2ml Control Invasion Inducer 1 x 300µl Wash Buffer 1 x 25ml
Images
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Standard curve
HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Invasion Dye and fluorescence (Em/Ex 485/530) was measured.
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Cell Invasion3T3-NIH and HT-1080 cells were starved overnight and treated with Control Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.
