Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Suspension cells
Overview
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Product name
Cell Invasion Assay (Basement Membrane), 24-well, 8 µm
See all Cell Invasion kits -
Detection method
Fluorescent -
Sample type
Suspension cells -
Species reactivity
Reacts with: Mouse, Human -
Product overview
This kit provides enough materials for 12 assays, i.e. to run 12 wells of assay, although it comes in a 24 well plate format.
Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882) utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
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Notes
Cell Invasion Assay (Basement Membrane), 24-well, 8 µm (ab235882) utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 12 tests Basement Membrane Solution 1 x 1.2ml Cell Dissociation Solution 1 x 6ml Cell Dye 1 x 1.5ml Cell Invasion Chamber 1 unit Control Invasion Inducer 1 x 300µl Wash Buffer 1 x 25ml
Images
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Standard Curve.
HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Invasion Dye and fluorescence (Em/Ex 530/590) was measured.
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Cell Invasion.3T3-NIH and HT-1080 cells were starved overnight and treated with Control Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.
