Anti-Pericentrin antibody - Centrosome Marker (ab4448)
Key features and details
- Rabbit polyclonal to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Pericentrin antibody - Centrosome Marker
See all Pericentrin primary antibodies -
Description
Rabbit polyclonal to Pericentrin - Centrosome Marker -
Host species
Rabbit -
Specificity
This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHuman
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Immunogen
Fusion protein corresponding to Mouse Pericentrin aa 100-600.
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Positive control
- ICC: Human coronary artery endothelial, MCF7, HeLa and NIH/3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Protein G purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF MouseHumanAll applications RatRabbitAfrican green monkeyApplication Abreviews Notes ICC/IF (27) Use a concentration of 0.1 - 0.5 µg/ml.Notes ICC/IF
Use a concentration of 0.1 - 0.5 µg/ml.Target
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Function
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
Tissue specificity
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
Involvement in disease
Microcephalic osteodysplastic primordial dwarfism 2 -
Domain
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
Cellular localization
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 5116 Human
- Entrez Gene: 18541 Mouse
- Omim: 605925 Human
- SwissProt: O95613 Human
- SwissProt: P48725 Mouse
- Unigene: 474069 Human
- Unigene: 251794 Mouse
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Alternative names
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Image courtesy of an anonymous abreview
Human coronary artery endothelial cells (HCAEC) were labeled for Pericentrin using ab4448 at 1/100 dilution in ICC/IF. Primary incubation was for 1 hour at room temperature (green). The seciondary antibody was a Goat anti-rabbit IgG AlexaFluor® conjugate.
Fixation: Formaldehyde. Permeabilisation: 0.1% Trition X-100 in HBSS.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.
Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).
Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Gordon Chan, University of AlbertaIF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.
The top panel is an interphase cell showing centrosome staining.
The bottom panel shows a mitotic cell with spindle pole staining.
ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).
Top panel - 630X magnification; Bottom panel -1000X magnification.
The secondary antibody was Alexa-Fluor®488 anti-rabbit.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia LaviaNIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.
The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in methanol (5 minutes) and incubated with the antibody (ab4448, 1 µg/ml) for 1 hour at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)ab4448 stained NIH/3T3 (Mouse embryo fibroblast cell line) cells.
The cells were fixed in 100% methanol for 5 minutes at -20°C and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References (360)
ab4448 has been referenced in 360 publications.
- Huhn O et al. Distinctive phenotypes and functions of innate lymphoid cells in human decidua during early pregnancy. Nat Commun 11:381 (2020). PubMed: 31959757
- Denu RA et al. Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Mol Cancer Res 18:33-45 (2020). PubMed: 31604847
- Naso FD et al. Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit. Cells 9:N/A (2020). PubMed: 32041138
- Mikulenkova E et al. NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation. Cells 9:N/A (2020). PubMed: 32168958
- Wu D & Dean J EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes. Nucleic Acids Res 48:5349-5365 (2020). PubMed: 32313933
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448) Image courtesy of an anonymous abreview
Human coronary artery endothelial cells (HCAEC) were labeled for Pericentrin using ab4448 at 1/100 dilution in ICC/IF. Primary incubation was for 1 hour at room temperature (green). The seciondary antibody was a Goat anti-rabbit IgG AlexaFluor® conjugate.
Fixation: Formaldehyde. Permeabilisation: 0.1% Trition X-100 in HBSS.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448) Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.
Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).
Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448) This image is courtesy of Gordon Chan, University of Alberta
IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.
The top panel is an interphase cell showing centrosome staining.
The bottom panel shows a mitotic cell with spindle pole staining.
ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).
Top panel - 630X magnification; Bottom panel -1000X magnification.
The secondary antibody was Alexa-Fluor®488 anti-rabbit.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448) This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.
The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in methanol (5 minutes) and incubated with the antibody (ab4448, 1 µg/ml) for 1 hour at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
ab4448 stained NIH/3T3 (Mouse embryo fibroblast cell line) cells.
The cells were fixed in 100% methanol for 5 minutes at -20°C and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.
-
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
