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Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
  • Suitable for: ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
    See all Pericentrin primary antibodies

  • Description

    Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    See all applications and species data

  • Immunogen

    Fusion protein corresponding to Mouse Pericentrin aa 100-600.

  • Positive control

    • ICC: HeLa cells.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    mAbcam 28144

  • Myeloma

    Sp2/0

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
    Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

    Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
    antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.

    Confocal image showing centrosome staining in HeLa cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) Image from Sikkema, William K. A. et al. PLoS ONE 9.6 (2014): e98838. PMC. Web. 24 Feb. 2017. doi: 10.1371/journal.pone.0098838. Fig 4F. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).

    This image was generated from the hybridoma version.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) This image is courtesy of an anonymous abreview.

    Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.

     This image was generated from the hybridoma version.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.

    This image was generated from the hybridoma version.

    See Abreview

  • Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
    Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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