Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

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Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4254] to HAUSP / USP7
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-HAUSP / USP7 antibody [EPR4254]
See all HAUSP / USP7 primary antibodies
Description

Rabbit monoclonal [EPR4254] to HAUSP / USP7
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human USP7 (UniProt Q93009).
Positive control
- WB: HEK-293T, T47-D and HeLa cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4254
Isotype

IgG
Research areas

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HAUSP / USP7 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF-7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109109 observed at 135 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109109 was shown to specifically react with HAUSP / USP7 when HAUSP / USP7 knockout samples were used. Wild-type and HAUSP / USP7 knockout samples were subjected to SDS-PAGE. ab109109 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)

All lanes : Anti-HAUSP / USP7 antibody [EPR4254] (ab109109) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : USP7 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 128 kDa
Observed band size: 128 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab109109 observed at 128 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab109109 was shown to react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266535 (knockout cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and USP7 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109109 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)

All lanes : Anti-HAUSP / USP7 antibody [EPR4254] (ab109109) at 1/1000 dilution

Lane 1 : T47-D cell lysate
Lane 2 : Hela cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 128 kDa
Observed band size: 135 kDa
why is the actual band size different from the predicted?
Anti-HAUSP / USP7 antibody [EPR4254] (ab109109)

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