Anti-HAUSP / USP7 antibody (ab4080)
Key features and details
- Rabbit polyclonal to HAUSP / USP7
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HAUSP / USP7 antibody
See all HAUSP / USP7 primary antibodies -
Description
Rabbit polyclonal to HAUSP / USP7 -
Host species
Rabbit -
Specificity
By Western blot, this antibody detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide. -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
TDDPPRITQNPVC
, corresponding to amino acids 30-41 of Human HAUSP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HAUSP / USP7 antibody (ab4080)
Western blot using ab4080 at 1/500. Secondary antibody anti-rabbit HRP ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41) -
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody (ab4080)ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ELISA - Anti-HAUSP / USP7 antibody (ab4080)ELISA results of purified ab4080 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Peroxidase Conjugated secondary antibody and TMB substrate.
