HRP Anti-polyHistidine antibody [HIS-1] (ab49781)
Key features and details
- HRP Mouse monoclonal [HIS-1] to polyHistidine
- Suitable for: ELISA, Dot blot, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-polyHistidine antibody [HIS-1] -
Description
HRP Mouse monoclonal [HIS-1] to polyHistidine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, Dot blot, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant polyHistidine tagged fusion protein.
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Positive control
- Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
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General notes
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Primary antibody notes
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors. -
Clonality
Monoclonal -
Clone number
HIS-1 -
Isotype
IgG2a -
Research areas