Antibodies, Proteins, Kits and Reagents for Life Science

Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR072G] to HAUSP / USP7
Suitable for: IP, WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-HAUSP / USP7 antibody [BLR072G]
See all HAUSP / USP7 primary antibodies
Description

Rabbit monoclonal [BLR072G] to HAUSP / USP7
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human HAUSP/ USP7 aa 1-50. The exact sequence is proprietary. NP_003461.2
Database link: Q93009
Positive control
- IHC-P: Human prostate carcinoma tissue. Mouse renal cell carcinoma tissue. ICC; OVCAR-4 cells. WB: RKO, HEK-293T, K562, HepG2, MCF7, Jurkat, HeLa, U-2 OS, NIH/3T3 and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 0.6% Boric Acid, 0.9% Sodium borate, 0.4% Sodium chloride
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR072G
Isotype

IgG
Research areas

Western blot - Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

All lanes : Anti-HAUSP / USP7 antibody [BLR072G] (ab264422) at 1/1000 dilution

Lane 1 : RKO whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : MCF7 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : U-2 OS whole cell lysate
Lane 9 : NIH/3T3 whole cell lysate
Lane 10 : TCMK-1 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 128 kDa

Exposure time: 3 seconds

Lysates prepared using NETN lysis buffer.
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 (Human ovarian cancer cell line) cells labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation - Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

HAUSP / USP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264422 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264422 at 1/1000 dilution

Lane 1: ab264422 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared using NETN lysis buffer.
Anti-HAUSP / USP7 antibody [BLR072G] (ab264422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com