Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)
![Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-349651-anti-alix-antibody-epr15314-33-n-terminal-western-blot-knockout-hek293-pdcd6ip-hela-human-lysates.jpg "Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15314-33] to ALIX - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ALIX antibody [EPR15314-33] - N-terminal
See all ALIX primary antibodies -
Description
Rabbit monoclonal [EPR15314-33] to ALIX - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, K562 and 293 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15314-33 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)All lanes : Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : ALIX knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab186728 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab186728 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in ALIX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ALIX knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab186728 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-220015-anti-alix-antibody-epr15314-33-n-terminal-western-blot.jpg)
Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)All lanes : Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa
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![Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)](https://www.abcam.com/ps/products/186/ab186728/Images/ab186728-3-benefits-of-recombinant-antibodies.png)
Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)
