Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)
![Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-7-anti-cd97-antibody-epr4427-bsa-and-azide-free-flow-cytometry.jpg "Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4427] to CD97 - BSA and Azide free
- Suitable for: ICC, IP, WB, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD97 antibody [EPR4427] - BSA and Azide free
See all CD97 primary antibodies -
Description
Rabbit monoclonal [EPR4427] to CD97 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247649 is the carrier-free version of ab108368. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247649 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4427 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)
This data was developed using ab108368, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of MDA-MB-231 (human mammary gland epithelial adenocarcinoma) cells labeling CD97 with purified ab108368 at 1/250 dilution (10ug/ml) (red). A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. -
![Immunocytochemistry - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-5-anti-cd97-antibody-epr4427-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)This data was developed using ab108368, the same antibody clone in a different buffer formulation.ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-4-anti-cd97-antibody-epr4427-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)This data was developed using ab108368, the same antibody clone in a different buffer formulation.ab108368 at 1/250 dilution staining CD97 in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-405899-anti-cd97-antibody-epr4427-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)All lanes : Anti-CD97 antibody [EPR4427] (ab108368) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 (Human histiocytic lymphoma cell line) cell lysate
Lane 4 : Human fetal thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 92 kDaThis data was developed using ab108368, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-2-anti-cd97-antibody-epr4427-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)This data was developed using ab108368, the same antibody clone in a different buffer formulation.ab108368 at 1/250 dilution staining CD97 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-1-anti-cd97-antibody-epr4427-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)This data was developed using ab108368, the same antibody clone in a different buffer formulation.ab108368 at 1/250 dilution staining CD97 in human colon adenocarcinoma tissue by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)](https://www.abcam.com/ps/products/247/ab247649/Images/ab247649-6-benefits-of-recombinant-antibodies.png)
Anti-CD97 antibody [EPR4427] - BSA and Azide free (ab247649)