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Anti-ALIX antibody (ab225555)

Anti-ALIX antibody (ab225555)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ALIX
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ALIX antibody
    See all ALIX primary antibodies
  • Description

    Rabbit polyclonal to ALIX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ALIX aa 818-868. The exact sequence is proprietary. (NP_037506.2).
    Database link: Q8WUM4

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: HeLa whole cell lysate (ab150035). IP: HeLa whole cell lysate (ab150035).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Images

  • Western blot - Anti-ALIX antibody (ab225555)
    Western blot - Anti-ALIX antibody (ab225555)
    All lanes : Anti-ALIX antibody (ab225555) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 96 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALIX antibody (ab225555)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALIX antibody (ab225555)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ALIX using ab225555 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • Immunoprecipitation - Anti-ALIX antibody (ab225555)
    Immunoprecipitation - Anti-ALIX antibody (ab225555)

    ALIX was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225555 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225555 at 0.4 µg/ml.

    Lane 1: ab225555 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ALIX antibody (ab225555)

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    Anti-ALIX antibody (ab88388)

    Applications: ICC, WB

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    Anti-ALIX antibody [3A9] (ab117600)

    Applications: WB

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    Anti-ALIX antibody [EPR15314-33] - BSA and Azide free (ab226768)

    Applications: WB

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    Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)

    Applications: WB

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    Anti-ALIX antibody (ab76608)

    Applications: IHC-P, WB

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    Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728)

    Applications: WB

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    Anti-ALIX antibody [EPR15314] - BSA and Azide free (ab232611)

    Applications: WB

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