Antibodies, Proteins, Kits and Reagents for Life Science

Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR072G] to HAUSP / USP7 - BSA free
Suitable for: IP, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-HAUSP / USP7 antibody [BLR072G] - BSA free
See all HAUSP / USP7 primary antibodies
Description

Rabbit monoclonal [BLR072G] to HAUSP / USP7 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HAUSP/ USP7 aa 1-50. The exact sequence is proprietary. NP_003461.2
Database link: Q93009
Positive control
- IHC-P: Human prostate carcinoma tissue. Mouse renal cell carcinoma tissue. ICC; OVCAR-4 cells. IP: HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272079 is the carrier-free version of ab264422. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR072G
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 (Human ovarian cancer cell line) cells labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue labeling HAUSP / USP7 with ab264422 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
Immunoprecipitation - Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

HAUSP / USP7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264422 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264422 at 1/1000 dilution
Lane 1: ab264422 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264422).
Anti-HAUSP / USP7 antibody [BLR072G] - BSA free (ab272079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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