PE Anti-NF-kB p65 antibody [E379] (ab208750)
![PE Anti-NF-kB p65 antibody [E379] (ab208750)](https://www.abcam.com/ps/products/208/ab208750/Images/ab208750-258056-ICCap00251861.jpg "PE Anti-NF-kB p65 antibody [E379] (ab208750)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [E379] to NF-kB p65
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-NF-kB p65 antibody [E379]
See all NF-kB p65 primary antibodies -
Description
PE Rabbit monoclonal [E379] to NF-kB p65 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HeLa cells. ICC/IF: HeLa cells
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General notes
Alternative versions available:
Anti-NF-kB p65 antibody [E379] (ab32536) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E379 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - PE Anti-NF-kB p65 antibody [E379] (ab208750)
ab208750 staining NF-kB p65 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208750 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
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![PE Anti-NF-kB p65 antibody [E379] (ab208750)](https://www.abcam.com/ps/products/208/ab208750/Images/ab208750-3-benefits-of-recombinant-antibodies.png)
PE Anti-NF-kB p65 antibody [E379] (ab208750)
