Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein antibody [EPR5119(2)]
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Amyloid Precursor Protein aa 650-750. The exact sequence is proprietary.
    Database link: P05067

  • Positive control

    • 293T, SH-SY5Y, U87 MG and Human fetal brain lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Amyloid Precursor Protein knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133588 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133588 was shown to recognize Amyloid Precursor Protein when Amyloid Precursor Protein knockout samples were used, along with additional cross-reactive bands. Wild-type and Amyloid Precursor Protein knockout samples were subjected to SDS-PAGE. ab133588 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Immunoprecipitation - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

    ab133588 (purified) at 1/20 immunoprecipitating amyloid precursor protein in human fetal brain (Lane 2). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/20000 dilution (purified) + Human fetal brain tissue lysate

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + SH-SY5Y cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 86 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    All lanes : Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : U87 MG cell lysate
    Lane 4 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100-120 kDa
    why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)
    Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

© 2021 Astana Biomed Group LLP. All rights reserved.