Antibodies, Proteins, Kits and Reagents for Life Science

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Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-Amyloid Precursor Protein antibody [EPR5119(2)]
See all Amyloid Precursor Protein primary antibodies
Description

Rabbit monoclonal [EPR5119(2)] to Amyloid Precursor Protein
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Amyloid Precursor Protein aa 650-750. The exact sequence is proprietary.
Database link: P05067
Positive control
- 293T, SH-SY5Y, U87 MG and Human fetal brain lysates
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 9.50 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5119(2)
Isotype

IgG
Research areas

Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Amyloid Precursor Protein knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab133588 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133588 was shown to recognize Amyloid Precursor Protein when Amyloid Precursor Protein knockout samples were used, along with additional cross-reactive bands. Wild-type and Amyloid Precursor Protein knockout samples were subjected to SDS-PAGE. ab133588 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

ab133588 (purified) at 1/20 immunoprecipitating amyloid precursor protein in human fetal brain (Lane 2). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/20000 dilution (purified) + Human fetal brain tissue lysate

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + HEK293 cell lysate at 20 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/2000 dilution (purified) + SH-SY5Y cell lysate at 20 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

All lanes : Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : U87 MG cell lysate
Lane 4 : Human fetal brain tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 86 kDa
Observed band size: 100-120 kDa
why is the actual band size different from the predicted?
OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Amyloid Precursor Protein antibody [EPR5119(2)] (ab133588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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