Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)
![Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)](https://www.abcam.com/ps/products/133/ab133509/Images/ab133509-282602-anti-amyloid-precursor-protein-antibody-epr5118-western-blot.jpg "Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5118] to Amyloid Precursor Protein
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Amyloid Precursor Protein antibody [EPR5118]
See all Amyloid Precursor Protein primary antibodies -
Description
Rabbit monoclonal [EPR5118] to Amyloid Precursor Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Human Amyloid Precursor Protein aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P05067 -
Positive control
- Human brain, Human heart and SHSY-5Y lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5118 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Amyloid Precursor Protein knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133509 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133509 was shown to recognize Amyloid Precursor Protein when Amyloid Precursor Protein knockout samples were used, along with additional cross-reactive bands. Wild-type and Amyloid Precursor Protein knockout samples were subjected to SDS-PAGE. ab133509 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)](https://www.abcam.com/ps/products/133/ab133509/Images/ab133509-147191-anti-amyloid-precursor-protein-antibody-epr5118-western-blot.jpg)
Western blot - Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)All lanes : Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509) at 1/1000 dilution
Lane 1 : Human brain
lysate
Lane 2 : Human heart lysate
Lane 3 : SHSY-5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
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![OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)](https://www.abcam.com/ps/products/133/ab133509/Images/ab133509-147190-anti-amyloid-precursor-protein-antibody-epr5118.jpg)
OI-RD Scanning - Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509) -
![Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)](https://www.abcam.com/ps/products/133/ab133509/Images/ab133509-4-benefits-of-recombinant-antibodies.png)
Anti-Amyloid Precursor Protein antibody [EPR5118] (ab133509)
