Anti-RAGE antibody (ab37647)
Key features and details
- Rabbit polyclonal to RAGE
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-RAGE antibody
See all RAGE primary antibodies -
Description
Rabbit polyclonal to RAGE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF RatWB MouseCowHuman
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Immunogen
Synthetic peptide:
KGAPKKPPQRLEWKLNTGRTC
, corresponding to amino acids 39-58 of Human RAGE -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAGE antibody (ab37647) This image is courtesy of an anonymous AbreviewAll lanes : Anti-RAGE antibody (ab37647) at 1/500 dilution
Lane 1 : HUVEC whole cell lysate
Lane 2 : EVC304 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 17,40 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The band at 52kDa is a tubulin loading control. -
Flow Cytometry - Anti-RAGE antibody (ab37647)RAW 264.7 cells were stained with anti-RAGE (1ug/million cells) for 1hr at 4 deg C followed by staining with anti-rabbit IgG-PE conjugate. Cells were analyzed by flow cytometry. Unstained cells or cells stained with secondary antibody alone are represented in the background -
Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab37647) This image was kindly supplied by Dr Fabien Gosselet by Abreviewab37647 at a 1/100 dilution staining RAGE in bovine endothelial cells by Immunocytochemistry/ Immunofluorescence. Fixed in PFA, permeabilized with Triton X-100. Blocked using 10% serum for 20 minutes at room temperature. Secondary used at 1/200 polyclonal Goat snti-rabbit conjugated to Alexa Fluor 568 (red). Nuclear staining (blue). -
Western blot - Anti-RAGE antibody (ab37647)All lanes : Anti-RAGE antibody (ab37647) at 1/2000 dilution
Lane 1 : Bovine lung extract
Lane 2 : Mouse lung extract
Lane 3 : HeLa cells expressing human soluble RAGE
Secondary
All lanes : anti-rabbit IgG alkaline phosphatase conjugate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Bovine RAGE is slightly larger than human and mouse RAGE - 44kDa compared with 42kDa -
Flow Cytometry - Anti-RAGE antibody (ab37647) This image is courtesy of an anonymous Abreviewab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.
Specimen tube 006 is negative control
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Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab37647)ICC/IF image of ab37647 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
