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Human APOC3 ELISA Kit (ab238268)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 235 pg/ml
  • Range: 0.7 ng/ml - 8 ng/ml
  • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human APOC3 ELISA Kit
    See all Apolipoprotein CIII kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Breast milk 8 2%
    Inter-assay
    Sample n Mean SD CV%
    Breast milk 3 0.8%

  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    235 pg/ml

  • Range

    0.7 ng/ml - 8 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 91% - 110%
    Milk 96 87% - 101%
    Urine 95 93% - 97%
    Serum 119 112% - 128%
    Cell culture media 91 85% - 98%
    Hep Plasma 108 102% - 114%
    EDTA Plasma 117 103% - 130%
    Cit plasma 105 95% - 116%
    Cerebral Spinal Fluid 101 86% - 101%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human APOC3 ELISA Kit (ab238268) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of APOC3 protein in cerebral spinal fluid, cit plasma, edta plasma, hep plasma, milk, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human APOC3 with 235 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human APOC3 ELISA Kit (ab238268)
    Other - Human APOC3 ELISA Kit (ab238268)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human APOC3 standard curve in Sample Diluent NS.
    Example of human APOC3 standard curve in Sample Diluent NS.
    The APOC3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native APOC3 in human breast milk (de-fatted), urine, cerebrospinal fluid (CSF), and HepG2 cell culture supernatant samples.
    Interpolated concentrations of native APOC3 in human breast milk (de-fatted), urine, cerebrospinal fluid (CSF), and HepG2 cell culture supernatant samples.
    The concentrations of APOC3 were measured in duplicates, interpolated from the APOC3 standard curves and corrected for sample dilution. Undiluted samples are as follows: breast milk 3.33%, urine 50%, CSF 4.44%, and HepG2 supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 188.8 ng/mL in neat breast milk, 2.895 ng/mL in neat urine, 105.5 ng/mL in neat cerebrospinal fluid, and 77.04 ng/mL in neat HepG2 cell culture supernatant.
  • Interpolated concentrations of native APOC3 in human serum, and plasma samples.
    Interpolated concentrations of native APOC3 in human serum, and plasma samples.

    The concentrations of APOC3 were measured in duplicates, interpolated from the APOC3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20,000, plasma (citrate) 1:20,000, plasma (heparin) 1:20,000, and plasma (EDTA) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 116.4 µg/mL in neat serum, 124.3 µg/mL in neat plasma (citrate), 118.8 µg/mL in neat plasma (heparin), and 168.4 µg/mL in neat plasma (EDTA).

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOC3 concentration was determined to be 111.9 µg/mL with a range of 52.19 – 314.5 µg/mL.

  • Sandwich ELISA - Human APOC3 ELISA Kit (ab238268)
    Sandwich ELISA - Human APOC3 ELISA Kit (ab238268)
    To learn more about the advantages of recombinant antibodies see here.

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