Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
![Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)](https://www.abcam.com/ps/products/225/ab225533/Images/ab225533-286082-anti-rage-antibody-epr16849-75-immunoprecipitation.jpg "Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16849-75] to RAGE - Low endotoxin, Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR16849-75] to RAGE - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat lung lysates. IP: Mouse lung whole cell lysate.
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General notes
ab225533 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16849-75 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
This IP data was generated using the same anti-RAGE antibody clone [EPR16849-75] in a different buffer formulation (cat# ab181293).
RAGE was immunoprecipitated from 1 mg of mouse lung whole cell lysate with ab181293 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab181293 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse lung whole cell lysate 10µg (Input).
Lane 2: ab181293 IP in mouse lung whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181293 in mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)](https://www.abcam.com/ps/products/225/ab225533/Images/ab225533-2-benefits-of-recombinant-antibodies.png)
Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
