Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free (ab247370)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR778(2)Y] to SHC (phospho Y427) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free
    See all SHC primary antibodies

  • Description

    Rabbit monoclonal [EPR778(2)Y] to SHC (phospho Y427) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247370 is the carrier-free version of ab68166. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247370 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free (ab247370)
    Western blot - Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free (ab247370)
    All lanes : Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166) at 1/500 dilution

    Lane 1 : A431 lysate
    Lane 2 : A431 lysate - EGF treated

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa (possible isoform), 52 kDa (possible isoform)



    This data was developed using ab68166, the same antibody clone in a different buffer formulation.

    Beta tubulin loading control is also shown.

  • Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free (ab247370)
    Anti-SHC (phospho Y427) antibody [EPR778(2)Y] - BSA and Azide free (ab247370)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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