Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9875(B)] to gamma Adaptin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free
    See all gamma Adaptin primary antibodies

  • Description

    Rabbit monoclonal [EPR9875(B)] to gamma Adaptin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249398 is the carrier-free version of ab167153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249398 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9875(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)

    This data was developed using ab167153, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: gamma Adaptin knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: Human fetal brain tissue lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab167153 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab167153 was shown to specifically react with gamma Adaptin when gamma Adaptin knockout samples were used. Wild-type and gamma Adaptin knockout samples were subjected to SDS-PAGE. ab167153 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    This data was developed using ab167153, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling gamma Adaptin with ab167153 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    Western blot - Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    All lanes : Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 91 kDa



    This data was developed using ab167153, the same antibody clone in a different buffer formulation.

     

  • Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)
    Anti-gamma Adaptin antibody [EPR9875(B)] - BSA and Azide free (ab249398)

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