Anti-IGF1 Receptor (phospho Y1162 + Y1163) antibody (ab5680)
Key features and details
- Rabbit polyclonal to IGF1 Receptor (phospho Y1162 + Y1163)
- Suitable for: WB
- Reacts with: Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-IGF1 Receptor (phospho Y1162 + Y1163) antibody
See all IGF1 Receptor primary antibodies -
Description
Rabbit polyclonal to IGF1 Receptor (phospho Y1162 + Y1163) -
Host species
Rabbit -
Specificity
Although exhibiting a preference for IR/IGF-1R, this antibody has been shown by both peptide competition and protein blotting to react with other dual phosphotyrosine motifs from proteins such as c-Met and Shc. -
Tested Applications & Species
See all applications and species dataApplication Species WB Chinese hamster
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Immunogen
Synthetic phosphopeptide derived from the region of Insulin Receptor/IGF1R that contains tyrosines 1162 and 1163. The corresponding residues in the IGF1R are 1135 and 1136.
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Positive control
- CHO-T cells transfected with a vector containing insulin receptor and stimulated with 100 nM insulin for 10 minutes at 37°C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1162 and 1163(tyrosines 1135 and 1136 for IGF1R). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF1 Receptor (phospho Y1162 + Y1163) antibody (ab5680)Peptide Competition: Extracts prepared from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (1) or stimulated with insulin (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4°C and incubated with the ab5680 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), the non-phosphorylated peptide corresponding to the phosphopeptide (3), or, a generic phosphotyrosine containing peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpY1162/1163] completely blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the activation of the insulin receptor upon stimulation with insulin.
Peptide Competition: Extracts prepared from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (1) or stimulated with insulin (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4°C and incubated with the ab5680 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), the non-phosphorylated peptide corresponding to the phosphopeptide (3), or, a generic phosphotyrosine containing peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpY1162/1163] completely blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the activation of the insulin receptor upon stimulation with insulin.
