Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19667] to IRAK-1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free
    See all IRAK-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19667] to IRAK-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251482 is the carrier-free version of ab208009. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251482 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)

    This data was developed using ab208009, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)

    Lane 2: IRAK-1 knockout HAP1 whole cell lysate (40 µg)

    Lane 3: HeLa whole cell lysate (40 µg)

    Lane 4: HEK293 whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab208009 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab208009 was shown to specifically react with IRAK-1 when IRAK-1 knockout samples were used. Wild-type and IRAK-1 knockout samples were subjected to SDS-PAGE. ab208009 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    All lanes : Anti-IRAK-1 antibody [EPR19667] (ab208009) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa
    Observed band size: 68,80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab208009, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1, 3, and 4: 1 minute; Lane 2: 30 seconds.

    There are two splice variants for IRAK-1, IRAK-1b and IRAK-1c,respectively. The product recognizes full length IRAK-1 (80 kDa) and IRAK-1c (68 kDa) (PMID: 16690127).

  • Immunocytochemistry - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    Immunocytochemistry - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    This data was developed using ab208009, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling IRAK-1 with ab208009 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows: -ve control 1: ab208009 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.
  • Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
    Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)

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