Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
![Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)](https://www.abcam.com/ps/products/251/ab251482/Images/ab251482-406446-anti-irak-1-antibody-epr19667-bsa-and-azide-free-western-blot.jpg "Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19667] to IRAK-1 - BSA and Azide free
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free
See all IRAK-1 primary antibodies -
Description
Rabbit monoclonal [EPR19667] to IRAK-1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251482 is the carrier-free version of ab208009. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251482 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19667 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
This data was developed using ab208009, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: IRAK-1 knockout HAP1 whole cell lysate (40 µg)
Lane 3: HeLa whole cell lysate (40 µg)
Lane 4: HEK293 whole cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab208009 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab208009 was shown to specifically react with IRAK-1 when IRAK-1 knockout samples were used. Wild-type and IRAK-1 knockout samples were subjected to SDS-PAGE. ab208009 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)](https://www.abcam.com/ps/products/251/ab251482/Images/ab251482-403442-anti-irak-1-antibody-epr19667-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)All lanes : Anti-IRAK-1 antibody [EPR19667] (ab208009) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 68,80 kDa why is the actual band size different from the predicted?This data was developed using ab208009, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1, 3, and 4: 1 minute; Lane 2: 30 seconds.
There are two splice variants for IRAK-1, IRAK-1b and IRAK-1c,respectively. The product recognizes full length IRAK-1 (80 kDa) and IRAK-1c (68 kDa) (PMID: 16690127).
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![Immunocytochemistry - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)](https://www.abcam.com/ps/products/251/ab251482/Images/ab251482-1-anti-irak-1-antibody-epr19667-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)This data was developed using ab208009, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling IRAK-1 with ab208009 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows: -ve control 1: ab208009 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution. -
![Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)](https://www.abcam.com/ps/products/251/ab251482/Images/ab251482-4-benefits-of-recombinant-antibodies.png)
Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free (ab251482)
