Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)
![Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)](https://www.abcam.com/ps/products/247/ab247350/Images/ab247350-397899-anti-insulin-receptor-phospho-y1185-antibody-ep351-2-y-bsa-and-azide-free-western-blot.jpg "Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP351(2)Y] to Insulin Receptor (phospho Y1185) - BSA and Azide free
- Suitable for: ICC, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free
See all Insulin Receptor primary antibodies -
Description
Rabbit monoclonal [EP351(2)Y] to Insulin Receptor (phospho Y1185) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects Insulin Receptor when phosphorylated at Tyrosine 1185.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247350 is the carrier-free version of ab62321 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247350 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP351(2)Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)All lanes : Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] (ab62321) at 1/500 dilution
Lane 1 : HepG2 cell lysate; untreated
Lane 2 : HepG2 cell lysate; Insulin treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size: 156 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using ab62321, the same antibody clone in a different buffer formulation.
Beta Tubulin has been included as a loading control.
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![Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)](https://www.abcam.com/ps/products/247/ab247350/Images/ab247350-2-benefits-of-recombinant-antibodies.png)
Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] - BSA and Azide free (ab247350)
