Recombinant human TIE2 protein (ab129039)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human TIE2 protein
See all TIE2 proteins and peptides -
Biological activity
Measured in a functional ELISA assay. When ab129039 is immobilized at 4µg/ml (100 µl/well), it binds recombinant Human Angiopoietin 2 with a linear range of 2 - 100ng/ml. -
Purity
> 90 % SDS-PAGE. -
Expression system
Insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPL EVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQA SFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPD ILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNH LCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQE GCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEM CDRFQGCLCSPGWQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKA SGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVW VCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFG DGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRR GEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSED DFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQ GEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITI RYKVQGKNEDQHVDVKIKNATIIQYQLKGLEPETAYQVDIFAENNIGSSN PAFSHELVTLPESQAPADLGGGKTRHHHHHH -
Predicted molecular weight
95 kDa including tags -
Amino acids
23 to 745 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Reconstitutionab129039 is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50µg/ml.
Images
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Functional Studies - Recombinant human TIE2 protein (ab129039)Functional ELISA to test the binding of sTIE-2 to its ligand Ang-2. Ang-2 was coated on the plate and increasing amounts of recombinant human soluble sTIE-2 was added.
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SDS-PAGE - Recombinant human TIE2 protein (ab129039)SDS-PAGE of recombinant human soluble TIE-2-His. The sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
