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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-TIE1 antibody [8D12B10] (ab201977)

Anti-TIE1 antibody [8D12B10] (ab201977)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8D12B10] to TIE1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-TIE1 antibody [8D12B10]
    See all TIE1 primary antibodies
  • Description

    Mouse monoclonal [8D12B10] to TIE1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TIE1 aa 350-650.
    Database link: P35590

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • TIE1 transfected HEK293 cell lysate. HepG2 cell lysate. Human liver cancer tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8D12B10
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Images

  • Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution + human recombinant TIE1 protein

    Predicted band size: 125 kDa

  • Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    All lanes : Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution

    Lane 1 : Non-transfected HEK293 cell lysate
    Lane 2 : TIE1 transfected HEK293 cell lysate

    Predicted band size: 125 kDa

  • Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    Western blot - Anti-TIE1 antibody [8D12B10] (ab201977)
    Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution + HepG2 cell lysate

    Predicted band size: 125 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12B10] (ab201977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12B10] (ab201977)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissues labeling TIE1 using ab201977 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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