Anti-TIE1 antibody [8D12B10] (ab201977)
Key features and details
- Mouse monoclonal [8D12B10] to TIE1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TIE1 antibody [8D12B10]
See all TIE1 primary antibodies -
Description
Mouse monoclonal [8D12B10] to TIE1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TIE1 aa 350-650.
Database link: P35590 -
Positive control
- TIE1 transfected HEK293 cell lysate. HepG2 cell lysate. Human liver cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D12B10 -
Isotype
IgG1 -
Research areas
Images
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Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution + human recombinant TIE1 protein
Predicted band size: 125 kDa
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All lanes : Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate
Lane 2 : TIE1 transfected HEK293 cell lysate
Predicted band size: 125 kDa
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Anti-TIE1 antibody [8D12B10] (ab201977) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 125 kDa
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissues labeling TIE1 using ab201977 at 1/200 dilution followed by DAB staining.