Anti-TIE1 antibody [8C9] (ab27851)
![Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/ab27851-38793-anti-tie1-antibody-8c9-flow-cytometry.jpg "Anti-TIE1 antibody [8C9] (ab27851)")
Key features and details
- Mouse monoclonal [8C9] to TIE1
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-TIE1 antibody [8C9]
See all TIE1 primary antibodies -
Description
Mouse monoclonal [8C9] to TIE1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified from cell culture supernatant by chromatography. -
Clonality
Monoclonal -
Clone number
8C9 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-TIE1 antibody [8C9] (ab27851)Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Western blot - Anti-TIE1 antibody [8C9] (ab27851)](https://www.abcam.com/ps/products/27/ab27851/Images/ab27851-232684-anti-tie1-antibody-8c9-western-blot.jpg)
Western blot - Anti-TIE1 antibody [8C9] (ab27851)All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2
Predicted band size: 125 kDa
