Anti-TIE1 antibody [8D12D2] (ab201986)
![Anti-TIE1 antibody [8D12D2] (ab201986)](https://www.abcam.com/ps/products/201/ab201986/Images/ab201986-243280-anti-tie1-antibody-8d12d2-immunohistochemistry.jpg "Anti-TIE1 antibody [8D12D2] (ab201986)")
Key features and details
- Mouse monoclonal [8D12D2] to TIE1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TIE1 antibody [8D12D2]
See all TIE1 primary antibodies -
Description
Mouse monoclonal [8D12D2] to TIE1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment corresponding to Human TIE1 aa 350-650 (internal sequence).
Database link: P35590 -
Positive control
- Human TIE1 (AA: 385-607) recombinant protein, TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cell lysate, HepG2 cell lysate, Human ovarian cancer tissues, Human kidney tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D12D2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12D2] (ab201986)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TIE1 with ab201986 at 1/200 dilution with DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12D2] (ab201986)](https://www.abcam.com/ps/products/201/ab201986/Images/ab201986-243279-anti-tie1-antibody-8d12d2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12D2] (ab201986)Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling TIE1 with ab201986 at 1/200 dilution with DAB staining.
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![Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)](https://www.abcam.com/ps/products/201/ab201986/Images/ab201986-243278-anti-tie1-antibody-8d12d2-western-blot.jpg)
Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 125 kDa
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![Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)](https://www.abcam.com/ps/products/201/ab201986/Images/ab201986-243277-anti-tie1-antibody-8d12d2-western-blot.jpg)
Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)All lanes : Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 125 kDa
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![Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)](https://www.abcam.com/ps/products/201/ab201986/Images/ab201986-243276-anti-tie1-antibody-8d12d2-western-blot.jpg)
Western blot - Anti-TIE1 antibody [8D12D2] (ab201986)Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution + Human TIE1 (AA: 385-607) recombinant protein
Predicted band size: 125 kDa
