Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free
    See all EPH A3+A4+A5 primary antibodies

  • Description

    Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4  phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cell lysate (untreated/treated with Sodium Orthovanadate). Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 1560µl H2O and then 40µl H2O2, vortex and incubate at RT for 5-10min. Serum-starve cells overnight, and treat with the freshly made Pervanadate for 20min at 37°C.

  • General notes

    Ab188171 is the carrier-free version of ab124881. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab188171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution (purified)

    Lane 1 : Untreated HEK293 cell lysate
    Lane 2 : HEK293 cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 110, 115 kDa
    Observed band size: 133 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124881, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110, 115 kDa



    This data was developed using ab124881, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    This data was developed using ab124881, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
    Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)

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