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Recombinant Human TIE2 protein (Fc Chimera) (ab54435)

Recombinant Human TIE2 protein (Fc Chimera) (ab54435)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Insect cells
  • Purity: > 90% SDS-PAGE
  • Suitable for: SDS-PAGE, WB, ELISA

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Description

  • Product name

    Recombinant Human TIE2 protein (Fc Chimera)
    See all TIE2 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    Source: Insect cells. Purity: > 90%, by SDS-PAGE and visualised by silver stain. Endotoxin level:
  • Expression system

    Insect cells
  • Accession

    Q02763
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPL EVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQA SFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPD ILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNH LCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQE GCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEM CDRFQGCLCSPGWQGLQCEREGIQRMTPKIVDLPDHIEVNSGKFNPICKA SGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVW VCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFG DGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRR GEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSED DFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQ GEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITI RYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSN PAFSHELVTRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMT KNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • Amino acids

      23 to 731
    • Additional sequence information

      Sequence = aa23-731 of TIE2 fused to the Fc part of human IgG1.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Constituent: PBS

  • Reconstitution
    The lyophilised sTIE-2/Fc is soluble in water and most aqueous buffers. The lyophilised sTIE-2/Fc should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

Images

  • SDS-PAGE - Recombinant Human TIE2 protein (Fc Chimera) (ab54435)
    SDS-PAGE - Recombinant Human TIE2 protein (Fc Chimera) (ab54435)

    SDS-PAGE of recombinant human soluble TIE-2/Fc produced from insect cells. The sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.

  • Western blot - Recombinant Human TIE2 protein (Fc Chimera) (ab54435)
    Western blot - Recombinant Human TIE2 protein (Fc Chimera) (ab54435)
    Anti-TIE2 antibody [Cl. 16] (ab24859) at 1 µg/ml + Recombinant Human TIE2 protein (Fc Chimera) (ab54435) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 2 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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