Anti-CD44 antibody [M007] - Extracellular domain (ab253275)
![Anti-CD44 antibody [M007] - Extracellular domain (ab253275)](https://www.abcam.com/ps/products/253/ab253275/Images/ab253275-343891-AntiCD44-antibody-M007--Extracellular-domain-immunocytochemistry-a431-human.jpg "Anti-CD44 antibody [M007] - Extracellular domain (ab253275)")
Key features and details
- Mouse monoclonal [M007] to CD44 - Extracellular domain
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD44 antibody [M007] - Extracellular domain
See all CD44 primary antibodies -
Description
Mouse monoclonal [M007] to CD44 - Extracellular domain -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type within Human CD44. The exact sequence is proprietary. (Proprietary antigen related to the extracellular region of human CD44 from the A431 epidermoid carcinoma cell line).
Database link: P16070 -
Positive control
- WB: A549 and MDA-MB-231 whole cell lysates. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M007 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)
Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling CD44 using ab253275 at 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)](https://www.abcam.com/ps/products/253/ab253275/Images/ab253275-343890-AntiCD44antibody-M007-Extracellular-domain-Western-blot-a549-mdamb231-human.jpg)
Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)All lanes : Anti-CD44 antibody [M007] - Extracellular domain (ab253275) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) cell lysate, native conditions
Lane 2 : A549 (human lung carcinoma cell line) cell lysate, denatured conditions
Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate, native conditions
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate, denatured conditions
Predicted band size: 81 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.