Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6557] to SUN2
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-SUN2 antibody [EPR6557]
See all SUN2 primary antibodies
Description

Rabbit monoclonal [EPR6557] to SUN2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human SUN2 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9UH99
Positive control
- WB: Human fetal muscle, Saos-2, HeLa, Jurkat and HepG2 lysates. IHC-P: Human lung and ovary tissues. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 5.43 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6557
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)

Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 80 kDa
Flow Cytometry - Anti-SUN2 antibody [EPR6557] (ab124916)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SUN2 (red) with ab124916 at a 1/30 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)

Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Mouse heart lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)

All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution

Lane 1 : HeLa cell Lysate
Lane 2 : Jurkat cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)

All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal muscle lysate
Lane 2 : Saos-2 lysate
Lane 3 : HeLa lysate
Lane 4 : Jurkat lysate
Lane 5 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 80 kDa
OI-RD Scanning - Anti-SUN2 antibody [EPR6557] (ab124916)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-SUN2 antibody [EPR6557] (ab124916)

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