Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUN2 antibody [EPR6557] (ab124916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6557] to SUN2
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SUN2 antibody [EPR6557]
    See all SUN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6557] to SUN2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SUN2 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9UH99

  • Positive control

    • WB: Human fetal muscle, Saos-2, HeLa, Jurkat and HepG2 lysates. IHC-P: Human lung and ovary tissues. Flow Cyt: HeLa cells.

  • General notes

     

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

    ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Flow Cytometry - Anti-SUN2 antibody [EPR6557] (ab124916)
    Flow Cytometry - Anti-SUN2 antibody [EPR6557] (ab124916)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SUN2 (red) with ab124916 at a 1/30 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

    ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An  HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Mouse heart lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

    ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution

    Lane 1 : HeLa cell Lysate
    Lane 2 : Jurkat cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (ab124916)

    ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    Western blot - Anti-SUN2 antibody [EPR6557] (ab124916)
    All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal muscle lysate
    Lane 2 : Saos-2 lysate
    Lane 3 : HeLa lysate
    Lane 4 : Jurkat lysate
    Lane 5 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 80 kDa

  • OI-RD Scanning - Anti-SUN2 antibody [EPR6557] (ab124916)
    OI-RD Scanning - Anti-SUN2 antibody [EPR6557] (ab124916)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SUN2 antibody [EPR6557] (ab124916)
    Anti-SUN2 antibody [EPR6557] (ab124916)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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