Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)
Key features and details
- Rabbit polyclonal to Cytokeratin 18 (phospho S52)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 18 (phospho S52) antibody
See all Cytokeratin 18 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 18 (phospho S52) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 52. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
A synthesized phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 52 (STSPFR)
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Positive control
- Hela cells and extracts from HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)Immunofluorescence analysis of HeLa cells, using ab63393 at a 1:500 dilution.
Left image untreated.Right image treated with phospho peptide. -
Western blot - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)All lanes : Anti-Cytokeratin 18 (phospho S52) antibody (ab63393) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells plus phospho peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.
