Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23701-79] to G-protein coupled receptor 30
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-G-protein coupled receptor 30 antibody [EPR23701-79]
See all G-protein coupled receptor 30 primary antibodies
Description

Rabbit monoclonal [EPR23701-79] to G-protein coupled receptor 30
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human placenta tissue lysate; HepG2 whole cell lysate. IHC-P: Human placenta and cerebrum tissue; Rat kidney tissue; Mouse placenta and cerebrum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23701-79
Isotype

IgG
Research areas

Western blot - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

All lanes : Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lu carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 42 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 28849224, 29137421, 23273253).

Low expression control: A549 (PMID: 23273253).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling G-protein coupled receptor 30 with ab260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human placenta (PMID:9367686). The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat kidney PMID:21354433. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse placenta. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab260033 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling G-protein coupled receptor 30 with 260033 at 1/200 (2.565 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab26003 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-G-protein coupled receptor 30 antibody [EPR23701-79] (ab260033)

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