Giemsa Stain Kit (ab150670)
Key features and details
- Sample type: Tissue sections
Overview
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Product name
Giemsa Stain Kit -
Sample type
Tissue sections -
Product overview
Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
Giemsa staining protocol summary:
- deparaffinize sections if necessary and rehydrate
- incubate with May-Grunwald solution for 5-7 min
- wash with PBS
- incubate with Giemsa solution for 10-15 min
- wash with PBS, and incubate in PBS for 5 min
- rinse with water and air dry
- clear with Xylene or similar, and mountOther products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Nuclei Blue/Violet Cytoplasm Light Blue Collagen Pale Pink Muscle Fibre Pale Pink Erythrocytes Gray, Yellow or Pink Rickettsia Reddish-Purple Helicobacter Pylori Blue Mast Cells Dark Blue with Red Granules Control Tissue: Blood Film. Any well fixed tissue.
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 100 tests Giemsa Stock Solution 1 x 500ml May Grunwald Stock Solution 1 x 500ml Phosphate Buffer solution (pH6.8) 1 x 500ml -
Research areas
Images
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ab150670 - Giemsa Stain Kit
ab150670 Giemsa Stain Kit staining formalin-fixed-paraffin embedded human bone marror.
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ab150670 - Giemsa Stain Kitab150670 (Giemsa stain) staining mast cells (deep pink) in formalin fixed paraffin embedded normal human colon.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab150670 - Giemsa Stain KitStaining using ab150670 - Giemsa Stain Kit (May-Grunwald).