Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6557] to SUN2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SUN2 antibody [EPR6557] - BSA and Azide free
    See all SUN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6557] to SUN2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    Ab232365 is the carrier-free version of ab124916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232365 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Flow Cytometry - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SUN2 (red) with ab124916 at a 1/30 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

    ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

    ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

    ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    OI-RD Scanning - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

    ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An  HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124916).

  • Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)
    Anti-SUN2 antibody [EPR6557] - BSA and Azide free (ab232365)

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