Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1602Y] to Cytokeratin 6 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free
    See all Cytokeratin 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR1602Y] to Cytokeratin 6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247600 is the carrier-free version of ab93279 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    All lanes : Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/2000 dilution (purified)

    Lane 1 : Human skin tissue lysate
    Lane 2 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab93279, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)

    This data was developed using ab93279, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 6 with purified ab93279 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunocytochemistry - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Immunocytochemistry - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)

    This data was developed using ab93279, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labelling Cytokeratin 6 with purified ab93279 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used. Control 1: Primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
  • Immunocytochemistry - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Immunocytochemistry - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    This data was developed using ab93279, the same antibody clone in a different buffer formulation.ICC/IF image of unpurified ab93279 stained A431 (Human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93279, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabiit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/10000 dilution (purified) + HaCaT (Human keratinocyte cell line) cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    This data was developed using ab93279, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/5000 dilution (unpurified) + A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

    Predicted band size: 60 kDa



    This data was developed using ab93279, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    This data was developed using ab93279, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cervical carcinoma tissue labelling Cytokeratin 6 with unpurified ab93279 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)
    Anti-Cytokeratin 6 antibody [EPR1602Y] - BSA and Azide free (ab247600)

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