Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-RCAS1 antibody [EPR16568] (ab200348)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16568] to RCAS1
Suitable for: ICC/IF, IP, WB
Reacts with: Human

Product name

Anti-RCAS1 antibody [EPR16568]
See all RCAS1 primary antibodies
Description

Rabbit monoclonal [EPR16568] to RCAS1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, 293 and T-47D whole cell lysates; Human fetal brain and fetal kidney lysates. ICC/IF: HEK293 and T-47D cells. IP: HEK293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16568
Isotype

IgG
Research areas

Western blot - Anti-RCAS1 antibody [EPR16568] (ab200348)

All lanes : Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood ) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24, 29 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] (ab200348)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (Human ductal breast epithelial tumor cell line) cells, labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on T47D cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows:
-ve control 1: ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] (ab200348)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows:
-ve control 1: ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-RCAS1 antibody [EPR16568] (ab200348)

Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/1000 dilution + T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24, 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RCAS1 antibody [EPR16568] (ab200348)

All lanes : Anti-RCAS1 antibody [EPR16568] (ab200348) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24, 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] (ab200348)

RCAS1 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200348 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab200348 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200348 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200348 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-RCAS1 antibody [EPR16568] (ab200348)

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