Anti-Cytokeratin 18 (phospho S33) antibody (ab75747)
Key features and details
- Rabbit polyclonal to Cytokeratin 18 (phospho S33)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 18 (phospho S33) antibody
See all Cytokeratin 18 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 18 (phospho S33) -
Host species
Rabbit -
Specificity
ab75747 detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 33. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).
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Positive control
- Human breast carcinoma tissue; extracts from A431 cells and EC-304 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75747 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 (phospho S33) antibody (ab75747)ab75747, at a 1/50 dilution, staining Cytokeratin 18 in paraffin embedded human breast carcinoma tissue by Immunohistochemistrytissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunizing peptide.
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Western blot - Anti-Cytokeratin 18 (phospho S33) antibody (ab75747)All lanes : Anti-Cytokeratin 18 (phospho S33) antibody (ab75747) at 1/500 dilution
Lane 1 : A431 cell extracts with immunising peptide
Lane 2 : A431 cell extracts
Lane 3 : EC-304 cell extracts
Predicted band size: 48 kDa
Observed band size: 48 kDa
